Coupling S-adenosylmethionine–dependent methylation to growth: Design and uses

Luo, Hao and Hansen, Anne Sofie L. and Yang, Lei and Schneider, Konstantin and Kristensen, Mette and Christensen, Ulla and Christensen, Hanne B. and Du, Bin and Özdemir, Emre and Feist, Adam M. and Keasling, Jay D. and Jensen, Michael K. and Herrgård, Markus J. and Palsson, Bernhard O. and Khosla, Chaitan (2019) Coupling S-adenosylmethionine–dependent methylation to growth: Design and uses. PLOS Biology, 17 (3). e2007050. ISSN 1545-7885

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Abstract

We present a selection design that couples S-adenosylmethionine–dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated.

Item Type: Article
Subjects: STM Open Press > Biological Science
Depositing User: Unnamed user with email support@stmopenpress.com
Date Deposited: 13 Jan 2023 11:15
Last Modified: 07 May 2024 05:08
URI: http://journal.submissionpages.com/id/eprint/17

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