Wang, Xiaona and Guo, Jisheng and Song, Yinsen and Wang, Qi and Hu, Shunan and Gou, Lingshan and Gao, Yinbo (2018) Decreased Number and Expression of nNOS-Positive Interneurons in Basolateral Amygdala in Two Mouse Models of Autism. Frontiers in Cellular Neuroscience, 12. ISSN 1662-5102
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Abstract
The basolateral amygdala (BLA) controls socio-emotional behaviors and is involved in the etiology of autism. We have recently shown that virtually every neuronal nitric oxide synthase (nNOS) positive cell is a GABAergic inhibitory interneuron in the mouse BLA. Here, stereology was used to quantify the number of nNOS-expressing interneurons in valproic acid (VPA)-exposed C57BL/6J (B6) and BTBR T+Itpr3tf/J (BTBR) mice models of autism. Additionally, the protein and mRNA levels of nNOS in the BLA were quantitatively assessed by western blot and qRT-PCR analysis, respectively. Our results showed the decreased number of nNOS interneurons in the BLA of animal models relative to autism. Consistently, nNOS was significantly reduced in the VPA-exposed and BTBR mice at both protein and mRNA levels. Together, these preliminary findings suggest that down-regulation of nNOS may be an attractive target for the pharmacological intervention in autism.
Item Type: | Article |
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Subjects: | STM Open Press > Medical Science |
Depositing User: | Unnamed user with email support@stmopenpress.com |
Date Deposited: | 01 Jun 2023 07:21 |
Last Modified: | 17 Oct 2024 04:02 |
URI: | http://journal.submissionpages.com/id/eprint/1399 |