Purification and Characterization of Cellulase from Termite Ametermes eveuncifer (Silverstri) Soldiers

Fagbohunka, B. S. and Okonji, R. E. and Adenike, Ayinla Zainab (2016) Purification and Characterization of Cellulase from Termite Ametermes eveuncifer (Silverstri) Soldiers. International Journal of Biology, 9 (1). pp. 1-9. ISSN 1916-9671

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Official URL: https://doi.org/10.5539/ijb.v9n1p1

Abstract

Cellulase enzyme was purified and characterized from termite soldiers (Ametermes eveuncifer) using 70% ammonium sulphate precipitation, ion exchange chromatography and affinity chromatography. The enzyme isolated had a specific activity of 5.04 U/mg with a percentage yield of 11.7%. The enzyme showed maximum activity at 500C and pH 8. The enzyme was not inhibited by Ba2+ at a concentration of 1mM and Pb2+ at 10 mM concentration but was inhibited by other metal ions at 1 mM and 10 mM concentrations of their salts (NaCl, KCl, MnCl2, and NiCl2),

Item Type:	Article
Subjects:	STM Open Press > Biological Science
Depositing User:	Unnamed user with email support@stmopenpress.com
Date Deposited:	20 May 2023 05:12
Last Modified:	15 Oct 2024 10:22
URI:	http://journal.submissionpages.com/id/eprint/1305

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